Fig. 1.

Presence of SV40 Tag-specific CTL in naïve TRAMP mice and their elimination following specific immunization: TCR Clonotyping of a SV40 Tag CTL line derived from immunized C57BL/6 mice and detection of Tag-specifc CTLs in TRAMP mice. a RT-PCR was employed to monitor the abundance of the Vβ5.2–Jβ 1.3 population in a C57Bl/6 CTL line. The size of the Vβ5.2–Jβ1.3 population was found to enlarge in a CTL from vac-mTag immunized mice compared to naïve mice, and was further enriched in a long-term culture. In contrast, this population was not amplified in CD8+ splenocytes from C57Bl/6 mice immunized with control vaccinia (V69). b RNA was extracted from fresh splenocytes, subjected to RT reactions using Cβ primers and then PCR reactions using Vβ5.2 and Jβ1.3 primers for 40 cycles. Two microliters of RT-PCR products were separated on a 2% agarose gel (top), transferred onto nylon membrane, and hybridized with ³²P-labled Jβ1.3 primer (bottom)