Fig. 3.

TRAMP Tag-specific CTLs undergo Fas-mediated apoptosis following SV40 Tag-specific immunization. a RT-PCR and Southern blot for Tag-specific TCR in C57Bl/6, TRAMP, and TRAMP-lpr/lpr mice (4–6 mice per group) before and after immunization. The Tag-specific TCR persisted in TRAMP-lpr/lpr mice after Tag immunization. b Real-time RT-PCR confirmed the elimination of Tag-specific TCR in TRAMP mice in responding to Tag immunization and demonstrated that the expression of Tag-specific TCR in TRAMP-lpr/lpr mice was significantly higher than that in TRAMP mice carrying wild-type Fas (P < 0.01) in response to Tag immunization