Fig. 4.
IFNγ-releasing activity is restored in CTLs from TRAMP-lpr/lpr mice. C57Bl/6, TRAMP, and TRAMP-lpr/lpr mice (5–8 mice per group) were immunized with 107 pfu of vac-mTag or control vaccinia per mouse (V69). Three weeks later, splenocytes were stimulated in vitro with mitomycin C-treated syngeneic Tag-expressing tumor cells (WT19) or mKSA cells. Data shown represent mean ± SEM of one representative experiment. Mouse IFN-γ ELISPOT assay indicated that the Tag-specific CTL in naïve TRAMP-lpr/lpr, but not in naïve TRAMP, was able to respond to Tag-specific activation