Table 1.
The effect of IFNγ on cytokine-dependent proliferation by native human AML cells: a summary of the results for 67 consecutive patients
| Exogenous cytokine | Number of patients with detectable proliferation in IFNγ-free controlsa | Statistical comparison of proliferative response (mean cpm ± standard error) | Number of patients with >20% inhibitionb | ||
|---|---|---|---|---|---|
| Cultures without IFNγ | Cultures with IFNγ | P valuec | |||
| None | 21 | 5,885±1,376 | 9,692±3,456 | NS | – |
| IL1RA | 25 | 5,768±1,113 | 5,169±1,519 | NS | – |
| IL1β | 40 | 11,911±3,353 | 6,060±1,679 | 0.001 | 16/40 |
| IL3 | 49 | 17,543±3,661 | 12,048±2,015 | 0.052 | 22/49 |
| SCF | 50 | 22,491±4,553 | 11,078±2,510 | <0.0005 | 36/50 |
| Flt3L | 53 | 15,107±3,252 | 12,313±1,914 | NS | – |
| GM-CSF | 48 | 13,554±3,099 | 7,929±1,425 | 0.039 | 23/48 |
| G-CSF | 49 | 16,037±3,716 | 9,672±1,912 | 0.015 | 25/49 |
Native human AML cells were cultured in serum-free medium and proliferation assayed as 3H-thymidine incorporation after 7 days. The results are presented as counts per minute (cpm)
NS no significance
aA total of 67 consecutive patients were examined, but only those patients with detectable proliferation (>1,000 cpm) either in the IFNγ-containing or corresponding IFNγ-free control were included in the statistical analysis
bThe column indicates the number of patients who showed an IFNγ-induced alteration corresponding to at least 2,000 cpm and exceeding 20% of the control response
cThe two-tailed Wilcoxon’s signed rank test was used for the statistical analysis