Fig. 3.

Expression of CML28 gene in MO/DCs and target cells detected by RT-PCR and agarose gel electrophoresis. Total RNA was isolated from these cell populations: rAAV/CML28-transduced DCs, mock DCs, and untreated DCs at 48 h after the third transduction. These samples were analyzed by RT-PCR for the appropriate sized product. The CML28 positive control was the RT-PCR product from K562 cell lines. Human mRNA for GAPDH was also amplified at the same time as an internal positive control (600 bp). Note that only RNA from cells transduced with rAAV/CML28 virus resulted in an appropriate RT-PCR-sized product (708 bp), whereas the other cells did not do so. Lane1 GAPDH, Lane 2 CML28. The characterization of HLA-A2 and CML28 expression on the target cells used in the assay was illustrated in Table 1