Fig. 5.

Intracellular flow cytometry assay for detection of IFNγ-expressing CD8 positive T lymphocytes in the stimulated PBMCs of donors. Two days after the third stimulation with variously treated DCs, an IFNγ production assay was performed on the PBMCs. Data of two different experiments (a and b), each utilizing a different donor, are shown here. IFNγ-producing CD8 positive T cells are indicated as percentage in the bidimensional flow cytometry diagram. Note that a substantial proportion of IFNγ-producing CD8 positive T cells was observed only in the T cell populations primed by rAAV/CML28-transduced DCs, but neither in those by mock DCs nor in those by untreated DCs