FIG. 2.

Schematic of system for vector delivery to murine tracheas. Tracheotomies were performed in the surgically exposed tracheas of anesthetized mice for ventilation (distal) and vector instillation (proximal). Vector was instilled into the proximal tracheostomy filling the region between the two tracheostomies, where it remained in contact with the epithelium for a defined period of time. Typically vector was instilled in two separate aliquots of 10 to 15 μl for 1 h each (total duration of incubation = 2 h). The respiratory efforts of the mice limited vector to the region between the two tracheostomies, which was confirmed by visualization through a dissecting microscope.