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. 1998 Nov;72(11):8861–8872. doi: 10.1128/jvi.72.11.8861-8872.1998

FIG. 4.

FIG. 4

Functional correction of transformed (CFT1) and primary CF airway epithelial cells without selection, using a VSV-G-pseudotyped vector (HIT-LCFSN). (A) Western blot of CF cells infected with HIT-LCFSN. Lanes: 1, uninfected CFT1 cells; 2, CFT1 cells transduced by HIT-LCFSN; 3, uninfected primary human airway epithelial cells; 4, primary human airway epithelial cells transduced with HIT-LCFSN. (B) 36Cl efflux assay showing greater loss of Cl− from CFT1 cells infected with HIT-LCFSN than from uninfected cells. (C) Cl− secretory response (ΔIeqForskolin) of HIT-LCFSN-infected primary CF airway epithelial cells on permeable substrates relative to cells infected with Ad-CFTR. The data for Ad-CFTR-mediated Cl− secretory responses are taken from reference 18 and our laboratory database of Ad-CFTR in this culture system.