Extended Data Figure 3. Validation of CRISPR KO and siRNA KD in Raw 264.7 macrophages.

(A) Western blots from Raw264.7 6hrs after treatment +/− 100U/ml msIFN-β. (B) Western blot of Raw264–7 after transfection with NSC or protein of interest siRNA siRNA pool (10μM) pool for 24hrs or 48hrs. (C) Cell viability was determined using alamarblue Cell Viability Reagent and calculated as a percentage compared to the NSC. Statistical analysis was performed using GraphPad Prism 9, using an unpaired T-test.