Fig. 5.

Adoptive transfer of wild-type cells into GKO mice. Adoptive transfer of wild-type splenocytes restored anti-tumor effects of IL-12 in GKO mice. a GKO mice were intravenously injected with or without 2.0 × 10⁸ splenocytes from wild-type mice and, 1 day later, hydrodynamically injected with either pCMV-IL-12 or pCMV. Mice were killed 4 days after plasmid injection. Yac1 lytic ability of hepatic mononuclear cells was expressed as the indicated effector and target ratios (E/T ratio). Experiments were done 3 times and representative data are shown. b and c GKO mice were intrasplenically injected with CT-26 cells and, 1 day later, intravenously injected with or without 2.0 × 10⁸ splenocytes from wild-type mice. Two days after CT-26 injection, mice were hydrodynamically injected with either pCMV-IL-12 or pCMV. b The levels of serum IFNγ 4 days after plasmid injection are expressed as mean and SD (n = 6/group). c Fourteen days after plasmid injection, mice were killed to examine liver tumor development by measuring liver weight. The results are indicated as mean and SD (n = 6/group). ND not detectable. *p < 0.01. Adoptive transfer of wild-type NK cells, but not non-NK cells, restored anti-tumor effects of IL-12 in GKO mice. d Wild-type splenocytes were purified into DX5⁺ cells and DX5⁻ cells. GKO mice were intravenously injected with 4.0 × 10⁶ whole mononuclear cells or DX5⁺ cells or DX5⁻ cells and, 1 day later, hydrodynamically injected with either pCMV-IL-12 or pCMV. Mice were killed 4 days after hydrodynamic injection. Yac1 lytic ability of hepatic mononuclear cells is expressed as the indicated effector and target ratios (E/T ratio). Experiments were done 3 times and representative data are shown. e and f GKO mice were intrasplenically injected with CT-26 cells and, 1 day later, intravenously injected with whole mononuclear cells, DX5⁺ cells or DX5⁻ cells (4.0 × 10⁶/mouse). Two days after CT-26 injection, mice were hydrodynamically injected with either pCMV-IL-12 or pCMV. e The levels of serum IFNγ are expressed as mean and SD (n = 6/group). f Fourteen days after plasmid injection, mice were killed to examine liver tumor development by measuring liver weight. The results are expressed as mean and SD (n = 6/group). ND not detectable. *p < 0.001