Fig. 4.

a MTT assay to reveal anti-proliferative effects to cultured OC-3-VGH ovarian cancer cells upon 48 h incubation with I negative control (adjusted to 100%); II human IgG (10 μg/ml); III goat anti-human IgG (10 μg/ml); IV RP215 (10 μg/ml); and V RP215 (20 μg/ml), respectively (*p < 0.05; ^†p > 0.05); b MTT assay to reveal anti-proliferative effects to cultured A549, HCT116, MCF7, MB-MDA-231, OVCAR-3, SK-OV-3, and OC-3-VGH cancer cell lines upon incubation with 10 μg/ml of RP215 Mab for 48 h. Relative absorbance at 560 nm was normalized as 100% with the negative control (NC) without treatment (*p < 0.05; ^†p > 0.05); and c Downregulation of mRNA expression of the related ribosomal proteins designated as P0, P1, P2, and L37, respectively, upon treatments of cancer cells in cell culture with RP215 (10 μg/ml) for 24 h (gray). Negative control without treatment was normalized to 100% (white). mRNA expression of GAPDH gene served as the internal negative control and normalized to 100% (all data are statistically significant at p < 0.05 with duplicated experiments). For statistical analysis in a and b, ANOVA tests were performed with significant differences defined at p < 0.05 for data presented with mean ± SD in duplicated experiments