Fig. 3.

Liver infiltrates dependent on CD137 stimulation are composed mainly of CD8+ T lymphocytes, while the intensity of the infiltrates is mitigated in transgenic animals with a single TCR (OT-1 mice). The indicated groups of mice (6 per group) were treated with three weekly doses of 150 μg of anti-CD137 mAb 2A and the liver leukocytes were purified with percoll gradients. a The total numbers of recovered liver leukocytes. b, c Individual absolute numbers (left) and percentage (right) of CD3+CD8+ (b) and T cells CD4+ (c). A representative dot plot of CD3/CD8 and CD3/CD4 double stainings of samples from WT mice treated with anti-CD137 mAb is presented next to the graphs. The set of experiments is representative of two similarly performed experiments. Similar results were obtained on repeated treatment with 1D8 anti-CD137 mAb instead of 2A