Fig. 3.

TGF-β₁ upregulates Gal-1 expression in LP07 and MCF-7 tumor cells. LP07 (a) and MCF-7 (b) cells were treated for 24 h with different concentrations (0.4 and 4 ng/ml) of TGF-β₁. Following treatments, cells were lysed and analyzed for Gal-1 expression by western blot. Equal amounts of protein cell lysates (30 μg) were resolved by 15% SDS-PAGE, electroblotted onto nitrocelulose membranes and probed with the anti-Gal-1 polyclonal Ab as described in Materials and methods. Equal loading was checked by incubating the membranes with an anti-β-actin monoclonal antibody. The immunoreactive protein bands indicated by the arrow (a, b) were semiquantified by densitometry using the Scion Image software. Data are representative of three independent experiments