Fig. 3.

Evolution of leukocyte subpopulations during metronomic CTX treatment. a Leukocyte, lymphocyte and CD3⁺ T lymphocyte counts in the blood of patients (n = 9, Table 1) before and 1 month after initiating metronomic CTX. b Counts of T and NK cell subsets in blood. Flow cytometry analyses allowed to assess the percentages of CD3⁺CD4⁺ and CD3⁺CD8⁺ lymphocytes and CD3^-CD56⁺ NK cells. Absolute numbers were calculated from whole blood cell enumeration in the nine patients before and 1 month after initiation of metronomic CTX. Statistical analyses used the Fisher’s exact method