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Schematic illustration of the different Nanobody-derived constructs
Bio biotinylation tag; Hinge N-terminal part of the long hinge region found in IgG3 HcAb’s; His6 hexahistidine tag; MSA21 cDNA encoding the anti-mouse serum albumin (MSA) Nanobody clone 21; Myc cMyc-derived epitope tag; pLacZ LacZ promoter; B BstEII site; N Not1 site; P Pst1 site; S Sfi1 site
