Fig. 2.

ELISpot assays of MAGE-1-specific (a) or MAGE-3-specific (b) T-cell precursors from the splenocytes of vaccinated mice. The C57BL/6 mice were vaccinated with NE(−), MHM and NE(MHM). The control group received PBS. Each mouse was immunized three times every 10 days via subcutaneous (s.c.) injection. Mice were terminated on tenth day after the last immunization and splenocytes were isolated. Data shown represent averaged results obtained from six mice ± SEM. All analysis were done in duplicates. One-way ANOVA was performed for statistical analysis. A value of p<0.01 was considered significant. Asterisks denote significantly different from PBS and hash denotes significantly different from MHM protein vaccine alone