Fig. 6.

The preventive and immunotherapy effect of the challenged B16-MAGE-1 melanoma (a, c) or the B16-MAGE-3 melanoma (b, d) with the tumor vaccine (n=10 mice/group). Data presented are mean ± SEM. a and b The tumor growths both in B16-MAGE-1 tumor model and in B16-MAGE-3 tumor model were delayed by MHM and NE (MHM), respectively. Vaccination with MHM nanovaccine significantly delayed tumor growth compared to vaccination with the MHM protein alone. Kaplan–Meier curves (c, d) were generated from survival data. c and d Survival time of mice was significantly prolonged in the mice vaccinated with the MHM and NE (MHM), and the later prolonged survival time more much than the MHM protein group