Fig. 3.
Effect of a therapeutic 47-LDA vaccine combined with IL-15 and IL-21 genes on NXS2 tumor growth in A/J mice. a Schematic representation of the immunization schedule. b A/J mice (n = 6–8) that were inoculated s.c. with 106 NXS2 tumor cells were immunized with sham plasmid (a), the 47-LDA construct alone (b), sham plasmid in combination with IL-15 and IL-21 genes (c), the P18MN/PADRE T helper and junctional sequence construct in combination with IL-15 and IL-21 plasmids (d), the 47-LDA-MIMIC together with IL-15 and IL-21 plasmids (e), and the entire 47-LDA construct in combination with IL-15 and IL-21 genes (f). Animals were examined daily until the tumor became palpable, after which tumor growth was monitored by measuring s.c. tumors once to thrice a week. Lines represent tumor growth in individual mice