Table 2.

Primers and conditions for real-time PCR

Gene	Primer (5′–3′)	Product length (bp)	Annealing temperature (°C)
IL-2	Forward AACTCACCAGGATGCTCACATTTA	148	60
	Reverse TCCCTGGGTCTTAAGTGAAAGTTT
IL-4	Forward ACTGCTTCCCCCTCTGTTCT	375	60
	Reverse CTCTGGTTGGCTTCCTTCAC
IL-6	Forward GTGGCTGCAGGACATGACAA	101	60
	Reverse TGAGGTGCCCATGCTACATTT
IL-10	Forward GTGATGCCCCAAGCTGAGA	138	60
	Reverse CACGGCCTTGCTCTTGTTTT
IL-12p40	Forward TGGAGTGCCAGGAGGACAGT	147	60
	Reverse TCTTGGGTGGGTCAGGTTTG
IL-18	Forward GCTTGAATCTAAATTATCAGTC	342	55
	Reverse GAAGATTCAAATTGCATCTTAT
IL-21	Forward ACGAGACCAAGGTCTAGCTC	291	55
	Reverse GACTTTAGTTGGGCCTTCTG
IFN-γ	Forward TCAGCTCTGCATCGTTTTGG	120	60
	Reverse GTTCCATTATCCGCTACATCTGAA
TNF-α	Forward TCTTCTCGAACCCCGAGTGA	151	60
	Reverse CCTCTGATGGCACCACCAG
TGF-β1	Forward CAGCAACAATTCCTGGCGATA	136	60
	Reverse AAGGCGAAAGCCCTCAATTT
Perforin	Forward ACATAGGCATCCACGGCAGC	205	60
	Reverse GGCAGCGAGTTTACCCAGGC
G3PDH	Forward ACCACAGTCCATGCCATCAC	452	64
	Reverse TCCACCACCCTGTTGCTGTA