Fig. 5.

FOXP3 mRNA analysis of CD4(+)CD25^high T cells. CD4(+)CD25^high T regs were separated by FACS sorting on CD4(+)CD25^high T cells in PBMCs from gastric cancer patients (stage III and IV). Total RNA was extracted from sorted CD4(+)CD25^high T cells (samples 1–4) or CD4(+)CD25int T cells (samples 5, 6) and RT-PCR analyze specific for FOXP3 mRNA was performed