Fig. 4.

HGF and VEGF expression from tumor-bearing mice following systemic administration of a HGF expressing plasmid. Normal and tumor-bearing mice untreated or treated with hepatocyte growth factor as described in Fig. 3a were sacrificed. a Western blot analysis of HGF and VEGF expression in thymuses from different animals shows the expression of these growth factors following injection of HGF plasmid in tumor-bearing mice. The blot was stripped and reprobed with β-actin. b Densitometric analysis of the different protein levels of thymic HGF and VEGF. Serum levels of HGF (c) and VEGF (d) were assessed by Western blot and ELISA respectively. Recombinant mouse HGF (rHGF) (25 ng) was used as positive control (c). Data are representative of one of three experiments