Fig. 1.

Schematic representation of the new LVs. The pTY linker (A) was modified to construct pTY-TRIP-WPRE (B) that includes cPPT/CTS and WPRE elements. C–E Mono-promoter LVs, F–I dual-promoter LVs, J bicistronic retroviral vector (pMFG-scTCR-IRES-EGFP, pMTE) with wild-type LTR encodes scTCR and EGFP. K pTY-EF-EGFP, unmodified mono-promoter LV encoding EGFP, LTR long terminal repeat, cPPT/CTS HIV-1 central polypurine tract (cPPT) and the central termination sequence (CTS), WPRE Woodchuck hepatitis posttranscriptional regulatory element, del U3 deletion of 3′U3, scTCR single-chain T cell receptor (scTCR) fused to mouse CD3ζ domain, scTCR-AGD-TmHz scTCR fused to human CD3ζ domain through the AGD linker. mscTCR mutated scTCR, TGL Herpes simplex virus thymidine kinase suicide gene (HSV-TK) and enhanced green fluorescence protein (EGFP) fusion gene, EF1α elongation factor 1α promoter, PGK human phosphoglycerate kinase promoter, MU3 U3 promoter region of the murine stem cell virus long terminal repeat