Fig. 2.

Expression of CD1a, CD1b, CD1c and CD1d protein by freshly isolated human liver mononuclear cells (MNC). a Flow cytometric evaluation of the expression of transfected CD1a, CD1b, CD1c and CD1d by the C1R cells used in the present study. b Representative flow cytometry histograms showing cell-surface (top) and intracellular (bottom) CD1a–d expression by human hepatic MNC. Mean fluorescence intensities of cell-surface (c) and intracellular (d) staining of MNC from five histologically normal liver samples (open circles) and five liver samples taken from patients with hepatic malignancy (closed circles) with mAbs specific for CD1a–d after subtraction of mean fluorescence intensities obtained using isotype-matched antibody controls