Fig. 2.

Sorting of Melan-A specific lymphocytes from different sources. HLA-A*0201/Melan-A-A27L monomers coated on magnetic beads were used to isolate Melan-A-specific populations from unstimulated PBMC, peptide stimulated PBMC and TIL in three melanoma patients. PE-conjugated tetramers were used to assess the purity of the sorted populations. Values indicate the percentage of cells labeled with tetramers after one or two rounds of sorting/amplification steps