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Avidities of Melan-A-specific sorted CTL lines for Melan-A peptides. The TAP-deficient cell line T2 expressing HLA-A*0201 was loaded with various concentrations of either the natural peptides Melan-A26–35 (filled square) or Melan-A 27–35, (open square) or the modified decapeptide A27L (filled circle). Lytic activity was evaluated at an E:T ratio of 10:1 by the classical 4-h 51Cr release assay
