Fig. 4.

Reactivity of Melan-A specific sorted populations towards melanoma cell lines: open square patient M180, (hatched bars) patient M138, filled square patient M199. For lysis experiment, effector and target cells were incubated at a 10:1 ratio. For cytokine production, effector and target cells were incubated at a 1:2 ratio in the presence of Brefeldin A, stained with anti-cytokine antibody and analyzed by flow cytometry (5 × 10³ cells)