Fig. 5.

Ku86 expression is elevated in the CTCL cell line HuT78 and partially located in lipid rafts. a Western blot analysis of whole cell lysates of HuT78 and control PBMCs (ctrl) reveals higher amounts of Ku86 and Lck in the cell line. b Western blotting of the lipid raft fractions (fractions 3–5) depicts a minor portion of Ku86 in the rafts of both HuT78 and control PBMC. c Localization of Ku86 in the lipid rafts has been confirmed by a second sucrose density gradient using the lipid raft fractions (3–5) of the first gradient. Both, Ku86 and Lck, appeared again in the lipid rafts fractions