Table 1.

Primer real-time RT-PCR analysis

Name	Forward primer	Annealing temperature (°C)	Product size(bp)
	Reverse primer
MT5-MMP	att agc tca cac ctg tcc act c	66	106
	gca agt aac aac ctc tct gtg c
Integrin α4	ggg aaa atg gaa agt gga aaa g	59	142
	caa tta ctc ttg gat ttg gc
HMBS	ggc aat gcg gct gca a	64	75
	ggg tac cca cgc gaa tca c

The table shows sequences of forward and reverse primers in 5′3′ notation, as well as the annealing temperature and the size of the PCR product. MT5-MMP, membrane-type 5–matrix metalloproteinase, HMBS hydroxymethylbilane synthase