Fig. 4.
AZA prevents tumor cell outgrowth in cultures of tumor-infiltrated CD11b cells. Whole CT-26 tumor cell suspension has been prepared as described in “Materials and methods”. Tumor-infiltrated CD11b cells were purified by positive selection using magnetic beads. Fresh-isolated CD11b cells were washed with PBS, enumerated, placed in six-well plates (2 × 106 per well) in complete medium and cultured in the presence of AZA (5 μM) and GM-CSF (20 ng/ml). Microphotographs were taken on day 7. Images were visualized using Leica light microscope with original magnification ×100. Arrows indicate the tumor cell clusters/spheres that appeared in untreated cultures of tumor-derived CD11b cells