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RP-HPLC profiles of eluted peptides extracted from murine C1498 cells with TFA or CP buffer. Peptides were eluted from the same sample of murine C1498 myelomonocytic cell lines using TFA (bold line) or CP (fine line) buffer. The pools of leukemia-associated peptides were fractionated by RP-HPLC on an ACN gradient for 80 min. The TFA-elution procedure generated greater diversity of peptides
