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Combined treatment of A498 kidney cancer cells with bv-sPLA2 and PtdIns(3,4)P2 inhibits phosphorylation of PKB/Akt. Cells were left untreated (C control), were treated with bv-sPLA2 (200 μg/ml), with PtdIns(3,4)P2 (200 μM) with EGF (50 ng/ml) or a combination thereof as indicated. Phosphorylation of serine 473 of PKB/Akt was normalised to total PKB/Akt levels and was quantitatively determined by specific ELISA. Shown are normalised mean values ± SEM of four independent experiments
