Fig. 6.

Combined treatment with PtdIns(3,4)P2 and bv-sPLA2 enhances the number of 7-AAD⁺ cells. Cells were left untreated (C control), were treated with bv-sPLA2 (100 μg/ml), with PtdIns(3,4)P2 (100 μM) or a combination thereof as indicated. Distribution of FSC/SSC-hi (blue) or FSC/SSC-low (orange) populations are displayed in scatters diagrams and corresponding percentages are shown in appropriate colours (left panel). Overall 7-AAD stainings are presented in histograms and percentages (right panel). 7-AAD staining of the FSC/SSC-hi population is depicted in blue, whereas the 7-AAD staining of the FSC/SSC-low population is presented in orange