Skip to main content
. 2006 Sep 1;56(5):627–640. doi: 10.1007/s00262-006-0220-0

Fig. 8.

Fig. 8

Precursor ion scan 241 of bv-sPLA2 products of PtdIns(3,4)P2 in a cell free system to detect lyso-PtdIns(3,4)P2 by attenuated in source fragmentation using piperidine. a Cell culture medium containing PtdIns(3,4)P2 only, b cell culture medium containing a mixture of bv-sPLA2 (10 μg/ml) and PtdIns(3,4)P2 (10 μM). Lyso-PtdIns(3,4)P2 observable at m/z 731, was detected by the precursor ion scans 241 with the corresponding loss of InsP head group. The lyso-PtdIns observable at m/z 571, was produced from an in-source fragmentation of the PtdIns(3,4)P2 and also from the C32:0 PtdIns used as ISTD (C32:0 PtdIns-ISTD). Other in source fragments are also observable, e.g. product ions seen at m/z 651 and 673 and products thereof are from in-source fragmentation of (M − H)- parent ion m/z 969 and at (M −0H + Na)- m/z 991, respectively