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Generation of lyso-PtdIns(3,4)P2
| Treatment | Cell-free lyso-PtdIns(3,4)P2 (μM) | A498 cells lyso-PtdIns(3,4)P2 (μM) |
|---|---|---|
| Control (medium only) | 0.43 | - |
| Control (cells + medium) | - | 0.08 |
| bv-sPLA2 | 0.50 | 0.1 |
| PtdIns(3,4)P2 | 0.11 | 0.2 |
| bv-sPLA2 + PtdIns(3,4)P2 | 2.06 | 0.98 |
Quantification of lyso-PtdIns(3,4)P2 (m/z 571) was based on comparing intensities of the lyso species with known concentration of the ISTD using a precursor ion scan 241. Relative response factors were not considered in these calculations
