Fig. 2.

Immune responses after allogeneic bone marrow transplantation. a A/J mice radiated at 700 rad and allografted (H2 K^b → H2K^a) with 5 × 10⁶ T cell depleted (TCD) BMC display full donor chimerism in the spleen. b At 4 weeks post allogeneic transplantation the chimeric spleens include small numbers of CD4⁺ and CD8⁺ T cells (n = 13) as compared to naïve A/J hosts (H2K^a, n = 7). c Cytotoxicity Neuro-2a cells (target) by splenocytes (effector) harvested at 4 weeks after allogeneic bone marrow transplantation (n = 6), as determined from LDH release. Cytolysis of lymphocytes from naïve B6 donors and B6 mice immunized with two intravenous injections of 3 × 10⁶ Neuro-2a cells (at 3 day intervals) are presented as controls (n = 5). d Proliferation of splenocytes from allografted mice (H2 K^b → H2K^a) in response to LPS in vitro, as determined from CFSE dilution (n = 4). Demonstrative for assays were performed in triplicates