Fig. 4.

Immune reconstitution after allogeneic BMT and F1 splenocyte infusion. a Spleen cellularity measured at weekly intervals in mice grafted with 5 × 10⁶ allogeneic TCD-BMC (Allo BMT) and with 2 × 10⁷ F1 (H2K^a/b) splenocytes on day 0 (Allo + F1 spln.), according to the experimental setting detailed in Fig. 1b. Spleen cellularity in naïve donors (B6) and recipients (A/J) is given as control. b Fractional distribution of CD4⁺ and CD8⁺ T cells in the spleen measured at weekly intervals after transplantation of TCD-BMC (Allo BMT) and co-transplantation of F1 splenocytes (Allo + F1 spln.). The distribution of these subsets in naïve H2K^a (A/J) recipients is shown as control. c Dissociation between splenocytes derived from the bone marrow (H2 K^b) and F1 splenocytes (H2K^a/b) at 1 and 2 weeks post-transplantation (upper panels). At 4 weeks post-transplantation (lower panels), the spleen was reconstituted from the bone marrow cells (CD45.1), without evidence of residual F1 splenocytes (CD45.2). d Switch in splenic contribution from adoptively transferred F1 splenocytes to bone marrow-derived cells during the first two weeks after F1 splenocyte in fusion