Fig. 3.

CTLs, induced by MPLA/IFNγ DCs, are CD45RA⁻, CCR7⁻, CD28⁺ CD27⁺ and GrB⁺ and secrete IFNγ upon antigen-specific stimulation. a The phenotype of the CTLs were analysed 7 days after the third stimulation by staining the CTLs for MART-1 TM and analysing the expression of CD27, CD28, CCR7 and CD45RA on the MART-1 TM positive and negative CTLs. b The CTLs of the same cultures were incubated with JY cells loaded with either Her2 peptide or MART-1 peptide in an E:T ratio of 2:1 for 5 h in the presence of BFA (10 μg/ml). Subsequently, the intracellular accumulation of IFNγ was determined by FACS. CD8⁺ cells are depicted. c CD8⁺ cells were also analysed for the presence of intracellular GrB in combination with MART-1 TM staining. A representative CTL culture out of multiple cultures of two independent experiments is shown