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Western blot assays with mAb 12H23 or mimotope-induced antibodies for EGFRvIII and EGFR recognition in Huh7-EGFRvIII and A431 cell lysates. EGFRvIII and EGFR detection using mAb 12H23 (lane 1, positive control) and the sera from mice immunized with the WHTEILKSYPHE-KLH conjugate (lane 2), the LPAFFVTNQTQD-KLH conjugate (lane 3), the control peptide-KLH conjugate (lane 4), or the carrier KLH alone (lane 5)
