Fig. 2.

Relative OVA specific IgG subclass responses to immunization. Individual sera collected from mice 1 week after the fourth immunization were analyzed by ELISA for specific IgG subclasses antibodies to OVA using a mouse subtyping kit. Each sample was diluted 1:20 in milk blocker, and then added to all the wells of a single row in a microtiter plate. Specific subclass secondary antibodies (goat) were then added to triplicate columns followed by biotinylated anti-goat (rabbit), and then extravidin:peroxidase. Reactions were stopped 2 min after the addition of substrate. Each bar represents the mean absorbance ± standard deviation of triplicate wells of sera collected from eight mice for all groups except OVA/Lip which consisted of six mice