Fig. 3.

Functional analysis of FoxP3⁺ CD4⁺ T cells. Cells were stimulated with PMA/ionomycin in the presence of monensin and stained for surface markers followed by intracellular staining for FoxP3 and IFN-γ. PBMC gated were CD3⁺, CD4⁺, and CD14^neg events. Quadrants show percentages of the CD4⁺ gate and are drawn according to isotype controls. a Healthy volunteer, b early stage patient having completed initial surgery and about to begin adjuvant chemotherapy, c early stage patient having completed adjuvant chemotherapy, d stage IV patient having not received chemotherapy for 30 days. Representative plots of three subjects from each donor group