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CTLs generated by Ub/HER-2 mini-gene vaccination are cytolytic. CTL cultures were generated from splenocytes of Ub/HER-2 mini-gene immunized mice restimulated 5 days in vitro with the different HER-2-derived peptides. Each CTL culture was tested against T2 cells (a) or MC-2 cells (b) pre-pulsed with relevant or irrelevant peptides or against MC-2 and MC-2/HER-2 tumor cells (c). One representative experiment out of three is shown, and mean ± SD of the triplicates is indicated
