Fig. 1.
Specificity and cytolytic capacity of antigen specific CTL clones. a The specificity and the cytotoxic potential of the MART-127–35 CTL clones were examined in a 51Cr-release assay measuring cell lysis of T2 cells pulsed with the specific (MART-127–35 and MART-126–35) peptide or an irrelevant peptide (HIV-1 pol476–484) in three E:T ratios. The peptide affinity of the MART-127–35 CTL clone was analyzed further in a 51Cr-release assay measuring cell lysis of T2 cells pulsed with different concentrations of the specific (MART-127–35, and MART-126–35) peptide at the E:T ratio 1:1. b Cytotoxic potential and peptide affinity of the Bcl-2208–217 CTL clone