Table 1.
Expression of MART-1 and class I HLA in tumor cell lines
| Cell line | MART-1 expressiona | HLA-A expressiona | HLA-A2 expressionb |
|---|---|---|---|
| FM9 | ND | ND | − |
| FM76 | ND | ND | ND |
| FM92 | ND | ND | + |
| ESTDAB-001 | +++ | ++ | ND |
| ESTDAB-006 | +++ | + | + |
| ESTDAB-007 | + | ++ | ++ |
| ESTDAB-014 | +++ | + | ++ |
| ESTDAB-017 | +++ | ND | ND |
| ESTDAB-019 | ++ | ++ | +++ |
| ESTDAB-021 | ++ | + | + |
| ESTDAB-026 | +++ | +++ | + |
| ESTDAB-027 | +++ | ++ | ND |
| ESTDAB-028 | +++ | ND | ++ |
| ESTDAB-029 | ++ | ND | + |
| ESTDAB-033 | +++ | ++ | + |
| ESTDAB-100 | +++ | ++ | ND |
| ESTDAB-117 | +++ | ND | ND |
| CAMA-1 | ND | ND | ND |
| HCT-166 | ND | ND | ND |
| MDA-MB-231 | ND | ND | ND |
| ZR-1-75 | ND | ND | – |
aData obtained from the ESTDAB-database (http://www.ebi.ac.uk/ipd/estdab)
bThe magnitude of HLA-A2 expression was determined using the staining index (SI) defined as MFIpositive − MFIbackground/2 × SDbackground where MFI is mean fluorescence intensity. SDbackground is the degree of spread in the negative population (unstained cells) to which the cells stained with HLA-A2 mAb is to be compared
+ 100 < SI < 2,000
++ 2,000 < SI < 3,000
+++ SI > 3,000