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Tumor cell apoptosis in vaccinated mice. Frozen sections from intracranial (b, d) and subcutaneous (a, c) GL261 tumors were analyzed for apoptotic cells by TUNEL assays as described. Mice receiving BMDC transfected with pVAX.hSVN(II–V) (c, d) and pVAX1 (a, b) are shown. All images are shown at ×40 magnification
