Fig. 1.

IFN-γ ELISPOT assays for peptide-reactive CD8+ T cells responses. Normal mice (N = 3) were immunized with the indicated dose of Eph-NPs, Eph + CFA, Eph peptide only (Eph) or NPs only (NPs), and killed on day 5 post-immunization. Spleen cells were harvested and CD8+ T cells isolated using CD8 MicroBeads as described in “Materials and methods”. CD8+ T cells were then subjected to IFN-γ ELISPOT assays to detect EphA2-derived peptide-specific CTLs. The data are represented as mean IFN-γ spots ± SD per 100,000 CD8+ T cells analyzed. Similar results were obtained in three independent experiments. *P < 0.05