Fig. 4.

Antigen-specific secretion of IFNγ is enhanced upon combined treatment with IL-15 and IL-21. Murine splenocytes were gp100/A2 TCR-transduced, cultured with cytokines and stimulated with B16 target cells as described in legend to Fig. 3. Stimulated T cells were analysed by commercial ELISA for the secretion of IFNγ (a), IL-2 (b) and IL-10 (c). During the stimulation assay, no exogenous cytokines were added to the T-cell:target cell co-cultures. T cells cultured with medium or Con A served as negative and positive controls, respectively. Mock-transduced T cells showed no cytokine secretion in any condition tested (data not shown). The different T-cell cultures and the levels of cytokines that were present in supernatants are indicated on the X- and Y-axis, respectively (mean ± SEM, n = 4 (IL-2 and IL-10) or n = 10 (IFNγ), *p < 0.05 and **p < 0.005 compared to IL-2)