Fig. 5.
a Cytokine profile of the HLA-DPB*0401/0402 restricted CD4+ clone 710–13 following activation by GV1001. Supernatants were harvested 24 h after stimulation of 50,000 T cells with 25,000 APC and GV1001 (1 μM) in serum-free medium. Seventy-five microliters was harvested from duplicate wells and analyzed using a Bio-rad Bio-Plex instrument. Results are expressed as mean concentration of cytokines (pg/ml) in the supernatants based on calculation from standard curves established with recombinant cytokines. b GM-CSF production by two CD4+ clones from patient no. 710. The GM-CSF production by 50,000 T cells from the cytotoxic clone 710–76 corresponds to 500 ng/106 T cells/24 h. Results were obtained as described in a