Fig. 1.

Antigen specificity of Aurora-A-reactive HTL clones. The specificity of the T cell clones was determined by their capacity to produce cytokine (GM-CSF or IFN-γ) using autologous irradiated PBMCs as APCs in the presence of various synthetic peptides (used at 10 μg/ml). HTL clones MT16, Sa11 and 4E (top panels) responded only to the Aurora-A_161–175 peptide (Aur-A₁₆₁) and clones 5, Sa24 and K22 (bottom row) only recognized peptide Aurora-A_233–247 (Aur-A₂₃₃). None of the clones displayed a significant response to the irrelevant aurora-A peptide or to an irrelevant peptide from the WT1 antigen (WT1_124–138). Columns means of triplicate determinations, bars SD. Columns without bars had SD < 10% the values of the mean