Fig. 6.
Direct recognition of Aurora-A-expressing tumor cells by Aurora-A161–175-reactive HTL clones. DR9-restricted HTL clone 4E (a) and DR53-restricted HTL clones Sa11 (b) and MT16 (c) were tested for their capacity to recognize antigen directly on Aurora-A+ tumor cells that are either matched or mismatched for the restricting MHC class II alleles. The MHC class II-negative Jurkat T cell lymphoma cells were also used as negative controls. The antigen specificity of these responses was demonstrated by blocking tumor recognition with anti-HLA-DR mAb (L243). Columns without bars had SD < 10% the values of the mean. Results are representative of two separate experiments