Fig. 8.

Aurora-A-reactive HTL recognize naturally processed antigen presented by transformed proliferating cells but not by normal dividing cells. a Western blot analysis to examine expression level of Aurora-A in normal PBMC, PHA-blasts, CD40-stimulated B cell blasts, T cell lymphomas (Jurkat, MT2) and EBV-LCLs. Anti-β-actin antibody was used as a loading control. b Direct recognition of Aurora-A-specific HTL clones 4E, 5 and Sa11 against CD40 activated B cells and Aurora-A-expressing EBV-LCLs with or without peptide. Columns means of triplicate determinations, bars SD. Columns without bars had SD < 10% the values of the mean. Results are representative of two separate experiments